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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
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Acetone, natural, ≥97%
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Sodium chloride solution, 0.85%
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(±)-Epichlorohydrin, analytical standard
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Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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Sodium chloride, 99.999% trace metals basis
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Piperidine, ReagentPlus®, 99%
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Acetone, ≥99%, meets FCC analytical specifications
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
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N,N-Dimethylformamide, analytical standard
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Diethyl ether, analytical standard
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Formaldehyde solution, tested according to Ph. Eur.
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Tetrahydrofuran, analytical standard
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(±)-Epichlorohydrin, purum, ≥99% (GC)
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Tetrahydrofuran, Selectophore, ≥99.5%
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Tetrahydrofuran, HPLC grade, ≥99.9%, inhibitor-free
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N,N-Dimethylformamide, anhydrous, 99.8%
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Diethyl ether
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Diethyl ether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
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Acetone, suitable for HPLC, ≥99.9%
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Diethylene glycol, ReagentPlus®, 99%
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1-Octanol, natural, ≥98%, FCC
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1-Octanol, ≥98%, FCC, FG
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Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
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N-Ethyldiisopropylamine, BASF quality, ≥98.0%
Supelco
Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
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Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
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Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
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Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O