SAB4200745

Anti-Protein A antibody, Mouse Monoclonal

clone SPA-27, purified from hybridoma cell culture

• Synonym(s): Anti-IgG binding protein A, Anti-SpA, Anti-Staphylococcal protein A, Anti-Staphylococcus ayreus protein A
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SPA-27, monoclonal
• form: buffered aqueous solution
• mol wt: ~42 kDa
• species reactivity: Staphylococcus aureus
• concentration: ~1.0 mg/mL
• technique(s): dot blot: suitable; flow cytometry: suitable; immunoblotting: suitable; immunofluorescence: suitable; immunoprecipitation (IP): 2.5-5 μg/test using purified protein A from Staphylococcus aureus; indirect ELISA: 0.25-0.5 μg/mL using 10 μg/mL of protein A from Staphylococcus aureus for coating. To reduce nonspecific binding we recommend on detection using a secondary anti-mouse, F(ab’)2 fragment antibody
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Staphylococcus aureus protein A, also known as SpA or IgG binding protein A, is a 42 kDa single chain polypeptide isolated from the cell wall of S. aureus Cowan I strain. It consists of a single polypeptide chain containing five repetitive immunoglobulin-binding domains, rich in aspartic and glutamic acids.Anti-Protein A antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SPA-27 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse.

Specificity

Monoclonal Anti-Protein A specifically recognizes Protein A from Staphylococcus aureus bacteria.

Immunogen

Protein A from Staphylococcus aureus Cowan I strain

Application

The antibody may be used in various immunochemical techniques including immunoblotting, dot blot, ELISA, immunofluorescence, immunoprecipitation and flow cytometry (FACS).

Biochem/physiol Actions

Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used either in its native form, conjugated to various markers or serve as a tag for a specific protein of interest. In addition, protein A has low affinity to mouse monoclonal antibodies from IgG1 isotype. Thus, a monoclonal anti-protein A antibody of the mouse IgG1 isotype has an advantage due to its specific targeting and binding to protein A via its Fab fragment. Monoclonal anti-protein A antibody can serve a useful tool for quantification and visualization of S. aureus bacteria and bacterial contaminations in different examined samples and for detection of protein A tagged proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the material safety data sheet (MSDS) for information regarding hazards and safe handling practices.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable