SAB4200721

Anti-GABA antibody, Mouse monoclonal

clone GB-69, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-Gamma-aminobutyric acid
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: GB-69, monoclonal
• form: buffered aqueous solution
• species reactivity: rat, monkey, frog, mouse, human, gerbil
• concentration: ~1.0 mg/mL
• technique(s): ELISA: suitable; dot blot: suitable; immunofluorescence: 5-10 μg/mL using human pancreatic tumor AsPC1 cell line; immunohistochemistry: 1-2.5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain and/or cerebellum sections
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GABRA1(2554) ; mouse ... Gabra1(14394) ; rat ... Gabra1(29705) ; rhesus monkey ... Gabra1(574302)

Description

General description

Anti-GABA antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GB-69 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified GABA conjugated to BSA. γ-Aminobutyric acid (GABA) is present in the central nervous system (CNS) of vertebrates. GABA is formed following enzymatic decarboxylation of L-glutamic acid by glutamic acid decarboxylase (GAD).

Immunogen

purified GABA conjugated to BSA

Application

Anti-GABA antibody, Mouse monoclonal has been used in:

• immunofluorescence
• immunohistochemistry
• enzyme linked immunosorbent assay (ELISA)
• dot-blot

Biochem/physiol Actions

γ-Aminobutyric acid (GABA) is the main inhibitory neurotransmitter. GABA mediates fast synaptic inhibition in the mature nervous system and plays multiple key roles as sensory circuits undergo functional development. GABA promotes proliferation of immune cells, embryonic stem cells and cortical progenitor cells. In addition, it is involved in protein synthesis and metabolism and plays a major role in the regulation of muscle tone, blood pressure, heart rate and respiration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable