902020

Desthiobiotin-PEG3-Azide

• Synonym(s): (4S,5R)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]-5-methyl-2-oxo-4-imidazolidinehexanamide
• Empirical Formula (Hill Notation): C₁₈H₃₄N₆O₅
• CAS Number: 1951424-99-9
• Molecular Weight: 414.50
• MDL number: MFCD28334534
• UNSPSC Code: 51171641
• NACRES: NA.22

Properties

• form: paste
• storage temp.: −20°C

Description

Application

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 10¹¹ vs. 10¹⁵ M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable