70122

Nutrient Broth

Liquid medium used for the cultivation of a wide variety of microorganism, NutriSelect^® Plus, suitable for microbiology

• Synonym(s): NB, standard nutrient broth, nutrient broth No.1, Standard - Nutrient Broth
• MDL number: MFCD01867738
• UNSPSC Code: 41171607
• NACRES: NA.74

Properties

• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: D(+)-glucose, 1 g/L ; peptone, 15 g/L ; sodium chloride, 6 g/L ; yeast extract, 3 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.5±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; life science and biopharma; microbiology
• storage temp.: 2-25°C
• suitability: Erysipelothrix spp.; nonselective for Brucella spp.; nonselective for Escherichia coli; nonselective for Listeria spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Nutrient Broth is a general-purpose fluid media containing yeast extract, peptone, sodium chloride and other basic nutritional requirements for the growth of a wide variety of microbes that don’t require specific nutrients. It is used in the routine cultivation of microorganisms from both clinical and non-clinical samples. Addition of different biological fluids like blood, serum, egg yolk etc. can make it suitable for cultivation of more fastidious microbes.

Application

•Nutrient Broth No. 1 is used for the routine cultivation of microorganisms not requiring specific nutritional requirements
•It is also used for the enumeration and enrichment of bacteria.
•Nutrient Broth No 1 can be used as a base for preparing special culture media.

Preparation Note

Dissolve 25 g in 1 litre distilled water and fill into tubes. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable