46345
Fiske-Subbarow reducer
Synonym(s):
Fiske-Subbarow reagent
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General description
formulation of 1-amino-2-naphthol-4-sulfonic acid, sodium sulfite and bisulfite according to C.H. Fiske and Y. Subbarow, J.Biol.Chem. 66, 375 (1925)
Application
The Fiske-Subbarow assay is a colorimetric assay designed to measure levels of inorganic phosphate in biological samples. Fiske-Subbarow reducer reagent is used as the reducer component of the Fiske-Subbarow assay.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1
Supplementary Hazards
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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E Durward et al.
BioTechniques, 25(4), 608-612 (1998-10-30)
Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method
Eduardo H S Sousa et al.
Analytical biochemistry, 348(1), 57-63 (2005-11-18)
We describe the development of a rapid colorimetric assay for soluble guanylate cyclase (sGC) activity adapted for a 96-well microplate. The assay greatly decreases the analysis time and cost over traditional methodologies based on radio- and immunoassays and high-performance liquid
A Kyaw et al.
Analytical biochemistry, 145(2), 230-234 (1985-03-01)
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of
The colorimetric determination of phosphorus
Fiske, C. H., Subbarow, Y.
The Journal of Biological Chemistry, 66, 375-375 (1925)
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