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Key Documents

WBLUF

Millipore

Immobilon Forte Western HRP substrate, 100 mL

Western blotting, dot/slot blotting

Synonym(s):

HRP substrate, chemiluminescent detection substrate, detection substrate

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

conjugate

peroxidase conjugate

Quality Level

200

packaging

pkg of 100 mL

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

Related Categories

General description

Premixed, ready to use chemiluminescent HRP detection reagent

Application

For use on immunoblots.
Western blotting, dot/slot blotting

Packaging

1 bottle for 1000 cm2

Quality

The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.

Storage and Stability

Stable for 1 year at room temperature from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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