MABN254

Anti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3

clone 6C3, from mouse

• Synonym(s): Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer′s disease amyloid protein, cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6C3, monoclonal
• species reactivity: human
• technique(s): dot blot: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_000475
• UniProt accession no.: P05067
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... APP(351)

Description

General description

Pan-amyloid-beta (1-40) is one of several functional peptides (39 to 43 amino acids in length) formed from proteolytic cleavage of the amyloid-beta precursor protein (APP) by the family of secretase enzymes. Although the APP protein is required for a number of biological functions, including axonal transport, cell adhesion, and transcription, the Ab 1-40 peptide has been studied mostly for its potential to aggregate and form neurite plaques, which may contribute to neurotoxicity, particularly in the context of neurodegenerative disease such as Alzheimer’s. Previous studies have demonstrated that Anti-pan amyloid beta peptide (MOAB2), clone 6C3 detects specifically amyloid-beta, but not APP. Furthermore MOAB2 detects multiple amyloid-beta 40 and amyloid-beta 42 conformations including unaggregated, oligomeric and fibrillar. In a number of immunohistochemical and biochemical analyses, MOAB2 consistently detected intraneuronal amyloid-beta, but not APP, and showed greater specificity to amyloid-beta than 6E10. MOAB2 is therefore suitable for sensitive and specific detection of accumulating amyloid-beta peptides in Alzheimer’s disease models.

Specificity

This antibody recognizes amyloid-ß and not APP (amyloid precursor protein). Specifically, clone 6C3 recognizes unaggregated, oligomeric, and fibrillar forms of Aß42 and Aß40.

Immunogen

Linear peptide corresponding to human pan amyloid beta peptide (MOAB-2).

Application

Anti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3 is an antibody against amyloid beta peptide (MOAB-2), pan, clone 6C3 for use in western blotting, IHC (Paraffin), Immunofluorescence, IP, Dot Blot.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Amyloid Beta Peptide in human Alzeimer′s diseased brain (see website for images).

Western Blot Analysis: A representative lot was used by an independent laboratory in unaggregated forms of Aß42 and Aß40. (Youmans, K.L., et al. (2012). Mol Neurodegener. 7;8.)

Dot Blot Analysis: Serial Aβ40 and Aβ42 dilutions were probed with MOAB-2 or 6E10 by an independent laboratory. (Youmans, K.L., et al. (2012). Mol Neurodegener. 7;8.)

Immunoprecipitationt Analysis: A representative lot was used by an independent laboratory in unaggregated and fibrillar forms of Aß42 and Aß40. (Youmans, K.L., et al. (2012). Mol Neurodegener. 7;8.)

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Immunohistochemistry in Alzheimer′s diseased human brain tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Amyloid Beta Peptide in human Alzheimer′s diseased brain tissue.

Target description

4 kDa calculated but could be larger since clone 6C3 recognizes unaggregated, oligomeric, and fibrillar forms of Aß42 and Aß40.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Alzheimer′s diseased human brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable