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Key Documents

20-265

Sigma-Aldrich

Dithiothreitol, 1M

Synonym(s):

2,3-Butanediol, 1,4-dimercapto-, Cleland′s reagent, Sputolysin

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About This Item

UNSPSC Code:
41116012
eCl@ss:
32160405
NACRES:
NA.41

form

solution

Quality Level

manufacturer/tradename

Upstate®

shipped in

dry ice

Application

Dithiothreitol, 1M may be used as a reducing agent in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer. It may also be used as a reagent in saliva sterilization.

Biochem/physiol Actions

Dithiothreitol (DTT), also known as Cleland′s reagent, is a reducing agent containing two thiol functional groups. It serves as a protective regent for thiol groups in protein chemistry studies. DTT is used in chemical peptide synthesis for reducing disulfide bonds in various substrates such as glutathione, proteins, and other macromolecules. It is also used as a mucolytic agent during sputum processing.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

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Sun Young Park et al.
Viruses, 12(12) (2020-12-11)
Although polyethylene glycol (PEG) application is the most widely used method in removing nonstructural proteins (NSPs) for foot-and-mouth disease (FMD) vaccine production, some NSPs remaining in the antigen could elicit antibodies against these proteins after repeated vaccinations in livestock. Therefore
A Novel Dental Caries Model Replacing, Refining, and Reducing Animal Sacrifice
Wolfoviz-Zilberman A, et al.
Applied Sciences, 11(15), 7141-7141 (2021)
I S Woolhouse et al.
Thorax, 57(8), 667-671 (2002-08-01)
Sputum analysis is used increasingly to assess airway inflammation in patients with chronic obstructive pulmonary disease, including those with chronic bronchitis and bronchiectasis. However, it is not known whether dithiothreitol (DTT), a reducing mucolytic agent regularly used to homogenise sputum
Reza Heidari et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 99, 1022-1032 (2018-01-09)
Cholestasis is a disorder characterized by impaired bile flow and accumulation of cytotoxic bile acids in the liver. On the other hand, oxidative stress and its deleterious consequences seem to have a significant role in cholestasis-induced organ injury. Hence, antioxidants
A Kr zel et al.
Journal of inorganic biochemistry, 84(1-2), 77-88 (2001-05-02)
D,L-Dithiothreitol (DTT), known also as Cleland reagent, is a thiol group protectant, used commonly in peptide and protein chemistry. Therefore, it is often added at high concentrations in preparations of proteins relevant to heavy metal biochemistry. The coordination of five

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