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HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si (25 cm Column)

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si (25 cm Column) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

581512-U

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

47763

Cholecalciferol (D3)

analytical standard

47775

Menadione (K3)

analytical standard

47783

(±)-α-Tocopherol

analytical standard

47785

(+)-γ-Tocopherol

analytical standard

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] hexane; [B] ethyl acetate

gradient

10 to 30% B in 10 min; held at 30% B for 2 min

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 290 nm

injection

10 μL

Description

Analysis Note

Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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