HPLC Analysis of Catechols and Resorcinols on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 20 mM phosphoric acid (pH 2.0 unadjusted): [B] acetonitrile; (75:25, A:B)

flow rate

1.5 mL/min

column temp.

30 °C

detector

UV, 270 nm

injection

25 μL

sample

as indicated in 20 mM phosphoric acid (pH 2.0)