GC Analysis of Basic Drugs on Equity-5

column

Equity-5, 30 m × 0.25 mm I.D., 0.25 μm (28089-U)

oven

45 °C (2 min), 25 °C/min to 110 °C, 15 °C/min to 200 °C, 6 °C/min to 280 °C (3 min)

inj. temp.

250 °C

MSD interface

325 °C

scan range

m/z = 40-450

carrier gas

helium, 40 psi for 0.2 min then 0.7 mL/min constant flow

injection

0.3 μL pulsed splitless @ 50 mL/min (0.5 min)

liner

2 mm I.D., splitless

sample

~15 ng on-column of a 16-component drug standard