- Novel function of Trim44 promotes an antiviral response by stabilizing VISA.
Novel function of Trim44 promotes an antiviral response by stabilizing VISA.
Journal of immunology (Baltimore, Md. : 1950) (2013-03-06)
Bo Yang, Jie Wang, Yanming Wang, Haiyan Zhou, Xiaodong Wu, Zhigang Tian, Bing Sun
PMID23460740
ABSTRACT
Virus-induced signaling adaptor (VISA) functions as a critical adaptor in the regulation of both the production of type I IFNs and the subsequent control of the innate antiviral response. In this study, we demonstrate that tripartite motif (Trim)44 interacts with VISA and promotes VISA-mediated antiviral responses. The overexpression of Trim44 enhances the cellular response to viral infection, whereas Trim44 knockdown yields the opposite effect. Trim44 stabilizes VISA by preventing VISA ubiquitination and degradation. These findings suggest that Trim44 functions as a positive regulator of the virus-triggered immune response by enhancing the stability of VISA.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
Sigma-Aldrich
Anti-Sp1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution