Skip to Content
Merck

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)
Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia
PMID25473981
ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS
Product Number
Brand
Product Description
95294
Sigma-Aldrich
Hydrogen peroxide solution, tested according to Ph. Eur.
88597
Millipore
Hydrogen peroxide solution, 3%, suitable for microbiology
44890
Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
40318
Sigma-Aldrich
Hydrogen peroxide solution, semiconductor grade ULSI PURANAL™ (Honeywell 17024), ≥30%
18304
Sigma-Aldrich
Hydrogen peroxide solution, 34.5-36.5%
IRMM524B
Iron, IRMM®, certified reference material, 0.5 mm wire
356808
Sigma-Aldrich
Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis
209309
Sigma-Aldrich
Iron, powder, −325 mesh, 97%
267945
Sigma-Aldrich
Iron, chips, 99.98% trace metals basis
95321
Supelco
Hydrogen peroxide solution, ≥30%, for trace analysis
F8263
Sigma-Aldrich
Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%
F8633
Sigma-Aldrich
Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
H1009
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
16911
Supelco
Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis
215422
Sigma-Aldrich
Iron(II) sulfate heptahydrate, ACS reagent, ≥99.0%
GF35663935
Iron, wire reel, 5m, diameter 0.025mm, as drawn, 99.99+%
GF05179709
Iron, wire reel, 1m, diameter 0.5mm, as drawn, 99.998%
GF02717502
Iron, wire reel, 0.5m, diameter 0.25mm, hard, 99.99+%
GF47408430
Iron, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.99+%
GF61040023
Iron, wire reel, 5m, diameter 0.25mm, hard, 99.99+%
GF96915134
Iron, wire reel, 50m, diameter 0.5mm, as drawn, 99.95%
GF81282135
Iron, wire reel, 2m, diameter 0.25mm, annealed, 99.99+%
GF82602176
Iron, wire reel, 0.05m, diameter 0.5mm, as drawn, 99.998%
GF08606606
Iron, rod, 500mm, diameter 12.7mm, as drawn, 99.95%
GF29558582
Iron, foil, 50x50mm, thickness 2mm, as rolled, 99.99+%
GF17042082
Iron, tube, 200mm, outside diameter 4.0mm, inside diameter 3.6mm, wall thickness 0.2mm, as drawn, 99.5%
GF62438520
Iron, rod, 200mm, diameter 25mm, as drawn, 98+%
GF61545674
Iron, tube, 100mm, outside diameter 8.0mm, inside diameter 5mm, wall thickness 1.5mm, annealed, 99.5%
GF85519299
Iron, foil, 300x300mm, thickness 1.0mm, as rolled, 99.5%
GF03803587
Iron, foil, 300x300mm, thickness 2.0mm, as rolled, armco« soft ingot 99.8%