"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

Proceedings of the National Academy of Sciences of the United States of America (1987-01-01)

M C Ledbetter, R J Beuhler, L Friedman

ABSTRACT

Cluster ions containing 50, 100, or 200 water molecules and one proton are accelerated to over 275 kV and impacted on thin carbon films on which tobacco mosaic virus has been dispersed. After bombardment the films are examined with a transmission electron microscope to study induced morphological alterations with respect to the energy transfer processes that take place during and after single cluster-ion impact. Observations on tobacco mosaic virus indicate that a single cluster impact results in the removal of a cylindrical segment of the virus. No fragments from the excised TMV segment were observed on the carbon foil. We speculate that cluster-ion bombardment may become a useful analytical technique for structural studies of large biological macromolecular complexes.

MATERIALS

Product Number

Brand

Product Description

GF23879009

Carbon, foil, 100x100mm, thickness 0.5mm, flexible graphite, 99.8%

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GF64932947

Carbon, foil, 10mm disks, thickness 0.2mm, flexible graphite, 99.8%

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Carbon, foil, 500x500mm, thickness 1.0mm, flexible graphite, 99.8%

GF13406633

Carbon, foil, 250x250mm, thickness 0.5mm, flexible graphite, 99.8%

GF57718503

Carbon, foil, 25x25mm, thickness 0.15mm, rigid graphite, fine grain size, 99.95%

GF42491291

Carbon, foil, 50x50mm, thickness 5.0mm, rigid graphite, medium grain size, 99.5%

GF99536338

Carbon, foil, 500x500mm, thickness 0.2mm, flexible graphite, 99.8%

GF95868365

Carbon, foil, 150x150mm, thickness 0.125mm, rigid graphite, fine grain size, 99.95%

GF71541031

Carbon, foil, not light tested, 150x150mm, thickness 0.075mm, flexible graphite, 99.8%

GF25155008

Carbon, foil, 50x50mm, thickness 2.5mm, rigid graphite, fine grain size, 99.95%

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Carbon, foil, 15x15mm, thickness 2.0mm, hOpg

GF85706400

Carbon, foil, 50x50mm, thickness 0.125mm, rigid graphite, fine grain size, 99.95%

GF87162857

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GF83177949

Carbon, foil, 12mm disks, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF78424735

Carbon, foil, 5x10mm, thickness 2.0mm, hOpg

GF37642822

Carbon, foil, 500x500mm, thickness 0.35mm, flexible graphite, 99.8%

GF36978104

Carbon, foil, 150x150mm, thickness 0.25mm, rigid graphite, fine grain size, 99.95%

GF42290939

Carbon, foil, not light tested, 150x150mm, thickness 0.125mm, flexible graphite, 99.8%

GF91624103

Carbon, foil, 12mm disks, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

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Carbon, foil, 25x25mm, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF02861782

Carbon, foil, not light tested, 300x300mm, thickness 0.075mm, flexible graphite, 99.8%

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Carbon, foil, 50x50mm, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

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Carbon, foil, 50x50mm, thickness 0.35mm, flexible graphite, 99.8%

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Carbon, foil, 25x25mm, thickness 0.5mm, pyrolytic graphite, 99.99%

GF08868314

Carbon, foil, 150x150mm, thickness 0.35mm, flexible graphite, 99.8%

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Carbon, foil, 250x250mm, thickness 1.0mm, flexible graphite, 99.8%

GF27179286

Carbon, foil, 150x150mm, thickness 1.0mm, flexible graphite, 99.8%

GF37688754

Carbon, foil, 25x25mm, thickness 0.5mm, rigid graphite, fine grain size, 99.997%