[Determination of cirsilineol in Herba Artemisiae scopariae collected in autumn by HPLC].

To establish a quantitative method for determination of cirsilineol in Herba Artemisiae Scopariae collected in Autumn. Sample was extracted with methanol, and cleaned up with polyamide column. ODS column was used with methanol-acetonitrile-0.5% acetic acid solution (24:19:57) as mobile phase. Detection wavelength was 347 nm. Cirsilineol in sample solution was well separated. Linearity of cirsilineol was good (r = 0.9998) in range of 0.1-0.8 microgram. The average recovery was 101.3%, RSD of repeatability was 3.04%. This method can be used for quality control of Herba Artemisiae Scopariae collected in autumn and its preparations.