- Thiamine pyrophosphate determination in whole blood and erythrocytes by high performance liquid chromatography.
Thiamine pyrophosphate determination in whole blood and erythrocytes by high performance liquid chromatography.
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition (1984-01-01)
A Floridi, M Pupita, C A Palmerini, C Fini, A Alberti Fidanza
PMID6500839
ABSTRACT
A sensitive method for the measurement of thiamine pyrophosphate in whole blood and erythrocytes, using a straight-phase high performance liquid chromatography (HPLC), is described. The method was based on sample extraction with trichloracetic acid, conversion of the vitamer to the corresponding high fluorescent thiochrome ester, and separation and measurement of the derivative by HPLC. Evidence for reproducibility and accuracy of the assay is presented. Data indicating the thiamine pyrophosphate levels to be expected in blood and erythrocytes of healthy humans are included.
MATERIALS