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  • First hyperpolarized [2-13C]pyruvate MR studies of human brain metabolism.

First hyperpolarized [2-13C]pyruvate MR studies of human brain metabolism.

Journal of magnetic resonance (San Diego, Calif. : 1997) (2019-10-28)
Brian T Chung, Hsin-Yu Chen, Jeremy Gordon, Daniele Mammoli, Renuka Sriram, Adam W Autry, Lydia M Le Page, Myriam M Chaumeil, Peter Shin, James Slater, Chou T Tan, Chris Suszczynski, Susan Chang, Yan Li, Robert A Bok, Sabrina M Ronen, Peder E Z Larson, John Kurhanewicz, Daniel B Vigneron
PMID31648132
ABSTRACT

We developed methods for the preparation of hyperpolarized (HP) sterile [2-13C]pyruvate to test its feasibility in first-ever human NMR studies following FDA-IND & IRB approval. Spectral results using this MR stable-isotope imaging approach demonstrated the feasibility of investigating human cerebral energy metabolism by measuring the dynamic conversion of HP [2-13C]pyruvate to [2-13C]lactate and [5-13C]glutamate in the brain of four healthy volunteers. Metabolite kinetics, signal-to-noise (SNR) and area-under-curve (AUC) ratios, and calculated [2-13C]pyruvate to [2-13C]lactate conversion rates (kPL) were measured and showed similar but not identical inter-subject values. The kPL measurements were equivalent with prior human HP [1-13C]pyruvate measurements.

MATERIALS
Product Number
Brand
Product Description
W6624
Pyruvic -2-13C acid, API for Clinical Studies, ≥99 atom % 13C, ≥95% (CP)