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  • Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems.

Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems.

Gene (1988-12-25)
E M Van Cott, G G Wilson
PMID3074019
ABSTRACT

Methyltransferase genes from the FnuDI, NaeI, NcoI, and XbaI restriction-modification systems have been isolated in Escherichia coli by 'shot-gun' cloning bacterial DNA fragments into plasmid vectors and selecting for protectively modified molecules that resist digestion by the corresponding restriction endonuclease.