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Discovery® RP-Amide C16 (5 µm) HPLC Columns

L × I.D. 25 cm × 3 mm, HPLC Column

Synonym(s):

RP-AmideC16 reversed-phase HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm

material

stainless steel column

Agency

suitable for USP L60

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

11% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 3 mm

surface area

200 m2/g

surface coverage

2.6 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical base material
fully porous particle

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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Features and Benefits

  • Excellent retention and resolution of polar compounds
  • Unique selectivity compared to C18
  • Excellent peak shape and efficiency
  • Less hydrophobic than C18 columns
  • Compatible with 100% aqueous mobile phases
   

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

Lot/Batch Number
290170-06
290170-05
290170-04
290170-03
290170-02

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Annamaria Jakab et al.
Journal of pharmaceutical and biomedical analysis, 27(6), 913-921 (2002-02-12)
Retention parameters of 45 different barbituric acid derivatives were determined on an amide embedded RP silica column (Discovery RP-AmideC16) using non-buffered water-acetonitrile eluent systems. Linear correlation were calculated between the logarithm of the capacity factor and the acetonitrile concentration in

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