Skip to Content
Merck
  • Rapid determination of pesticide residues in fruits and vegetables, using ultra-high-performance liquid chromatography/time-of-flight mass spectrometry.

Rapid determination of pesticide residues in fruits and vegetables, using ultra-high-performance liquid chromatography/time-of-flight mass spectrometry.

Food chemistry (2014-08-31)
P Sivaperumal, P Anand, L Riddhi
ABSTRACT

A multiresidue method, based on the sample preparation by solid-phase extraction cartridges and detection by ultra-high-performance liquid chromatography/time-of-flight mass spectrometry (UHPLC/TOF-MS), was used for the analysis of 60 pesticides in vegetable and fruit samples. Quantitation by UHPLC/TOF-MS is accomplished by measuring the accurate mass of the protonated molecules [M+H](+). The mass accuracy typically obtained is routinely better than 2ppm. The rates of recovery for pesticides studied were satisfactory, ranging from 74% to 111% with a relative standard deviation (RSD) of less than 13.2%, at concentrations below 10μgkg(-1). The method limit of quantification (MLOQ) for most compounds was below the MRLs established by the Food Safety Standard Authority of India and the European Union. The uncertainty was determined using repeatability, recovery and calibration curves data for each pesticide. The method illustrated is suitable for routine quantitative analyses of pesticides in food samples.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Carbon, mesoporous
Sigma-Aldrich
Carbon, mesoporous, nanopowder, graphitized, less than 250 ppm Al, Ti, Fe, Ni, Cu, and Zn combined
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
Sodium sulfate, tested according to Ph. Eur., anhydrous
Sigma-Aldrich
Sodium sulfate, BioUltra, anhydrous, ≥99.0% (T)
Supelco
Methanol, analytical standard
Sigma-Aldrich
Sodium sulfate, ≥99.99% trace metals basis
Sigma-Aldrich
Sodium sulfate, BioXtra, ≥99.0%
Sigma-Aldrich
Sodium sulfate, ≥99.0%, suitable for plant cell culture
Supelco
Buprofezin, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Carbon, mesoporous, hydrophilic pore surface
Sigma-Aldrich
Activated Charcoal Norit®, Norit® GAC 1240W, from coal, for potable water processing, steam activated, granular
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Methanol, Absolute - Acetone free
Sigma-Aldrich
Sodium sulfate, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99%
Sigma-Aldrich
Sodium sulfate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Methanol, ACS spectrophotometric grade, ≥99.9%
Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Activated Charcoal Norit®, Norit® RB3, for gas purification, steam activated, rod
Sigma-Aldrich
Activated Charcoal Norit®, Norit® PK 1-3, from peat, steam activated, granular
Sigma-Aldrich
Sodium sulfate, ACS reagent, ≥99.0%, anhydrous, granular
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed
Sigma-Aldrich
Activated Charcoal Norit®, Norit® CA1, wood, chemically activated, powder
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Methanol, Laboratory Reagent, ≥99.6%
Sigma-Aldrich
Sodium sulfate, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, ≥99%
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Methanol, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%