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B7389

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:20,000

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rabbit IgG (whole molecule)-Biotin antibody is specific for rabbit IgGs. IgG antibody plays a crucial role in humoral immune responses such as placental transport, complement activation, phagocytosis and cell surface-receptor binding.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule)-Biotin antibody is suitable for use in western blot using extracellular matrix protein extracts from the bone marrow of mice. The product can also be used for direct ELISA (1:20,000).
Anti-rabbit IgG (whole molecule)–biotin antibody can be used in enfragment analysis to visualize native GFP protein in stem cell tumours. This antibody can also be used in western blotting and immunohistochemistry. Further this can be used as a secondary antibody in quantified immunocytochemistry experiments. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically with all rabbit immunoglobulins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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C L Vituri et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 33(8), 889-895 (2000-08-02)
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein
Jerónimo Auzmendi et al.
Pharmaceuticals (Basel, Switzerland), 11(1) (2018-02-22)
Sudden unexpected death in epilepsy (SUDEP) is the major cause of death in those patients suffering from refractory epilepsy (RE), with a 24-fold higher risk relative to the normal population. SUDEP risk increases with seizure frequency and/or seizure-duration as in
Hongyun Li et al.
Bioscience reports, 36(2) (2016-01-23)
ATP-binding cassette transporter A7 (ABCA7) is highly expressed in the brain. Recent genome-wide association studies (GWAS) have identified ABCA7 single nucleotide polymorphisms (SNPs) that increase Alzheimer's disease (AD) risk, however, the mechanisms by which ABCA7 may control AD risk remain
Oskar Hammar et al.
BMC pregnancy and childbirth, 13, 201-201 (2013-11-07)
Prior reports suggest a link between gonadotropin-releasing hormone (GnRH) and gastrointestinal function. The aim of the study was to prospectively investigate women subjected to in vitro fertilization (IVF) using the GnRH analog buserelin, taking into account gastrointestinal symptoms and antibody
Bodil Roth et al.
Drug target insights, 8, 45-50 (2014-12-03)
Gonadotropin-releasing hormone (GnRH) 1 and 2 and luteinizing hormone (LH) receptors have been described in the gastrointestinal tract. We have previously demonstrated antibodies in serum against GnRH1 in patients with gastrointestinal dysfunction and diabetes mellitus, and antibodies against GnRH receptor

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