HPLC Analysis of Drugs of Abuse on Ascentis^® Express F5

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water

gradient

held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min

flow rate

0.3 mL/min

pressure

175 bar

column temp.

35 °C

detector

ESI(+), m/z 100-1000

injection

1 μL

sample

1 μg/mL in 50:50, water: methanol