跳转至内容

In vitro thrombolytic activity of purified streptokinase extracted from Streptococcus equinus VIT_VB2 isolated from bovine milk.

In vitro thrombolytic activity of purified streptokinase extracted from Streptococcus equinus VIT_VB2 isolated from bovine milk.

Journal of thrombosis and thrombolysis (2014-06-06)

Vaishnavi Babu, C Subathra Devi

摘要

Streptokinase (SK) is an extracellular enzyme secreted by various strains of β-hemolytic Streptococci. The main focus of the current study is to evaluate the in vitro thrombolytic activity of purified SK extracted from Streptococcus equinus VIT_VB2 (Accession no. JX406835) isolated from milk sample. The growth rate of S. equinus VIT_VB2 strain was studied with pH and biomass content which has positive significant effect on enzyme yield. A temperature of 10 °C and pH of 6 was found to be optimum for maximum SK activity. The specific activity of the purified SK produced by VIT_VB2 strain was found to be 6,585 IU mg(-1). The molecular mass of the enzyme was determined as 47 kDa by SDS-PAGE. In vitro thrombolytic activity of purified SK was determined using synthetic chromogenic substrate S-2251, the activity of the purified enzyme was found to be 6,330 ± 2.2 IU. The purity of SK was compared with standard SK by HPLC. This is the first report which reveals the SK activity of S. equinus isolated from milk sample.

材料

货号

品牌

产品描述

Sigma-Aldrich

乙腈, suitable for HPLC, gradient grade, ≥99.9%

Sigma-Aldrich

乙腈, HPLC Plus, ≥99.9%

Sigma-Aldrich

氢氧化钠, ACS reagent, ≥97.0%, pellets

Sigma-Aldrich

氢氧化钠, reagent grade, ≥98%, pellets (anhydrous)

Sigma-Aldrich

2 mol/L 氢氧化钠溶液溶液, 50% in H₂O

Sigma-Aldrich

无水碳酸钠, powder, ≥99.5%, ACS reagent

Sigma-Aldrich

硫酸铵, ACS reagent, ≥99.0%

Sigma-Aldrich

乙酸钠, anhydrous, ReagentPlus^®, ≥99.0%

Sigma-Aldrich

无水碳酸钠, ACS reagent, anhydrous, ≥99.5%, powder or granules

Sigma-Aldrich

乙腈, ACS reagent, ≥99.5%

Sigma-Aldrich

2 mol/L 氢氧化钠溶液溶液, BioUltra, for molecular biology, 10 M in H₂O

Sigma-Aldrich

乙腈, for HPLC, for UV, ≥99.9% (GC)

Sigma-Aldrich

氯化钠, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Sigma-Aldrich

乙酸钠, ACS reagent, ≥99.0%

Sigma-Aldrich

硫酸铵, for molecular biology, ≥99.0%

Sigma-Aldrich

乙腈, suitable for HPLC, gradient grade, ≥99.9%

Sigma-Aldrich

乙酸钠, puriss. p.a., ACS reagent, reag. Ph. Eur., anhydrous

Sigma-Aldrich

氢氧化钠, BioXtra, ≥98% (acidimetric), pellets (anhydrous)

Sigma-Aldrich

氢氧化钠, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets

Sigma-Aldrich

2 mol/L 氢氧化钠溶液溶液, 1.0 N, BioReagent, suitable for cell culture

Sigma-Aldrich

氯化钠溶液, 0.9% in water, BioXtra, suitable for cell culture

Sigma-Aldrich

氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Sigma-Aldrich

氯化钠溶液, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

氢氧化钠, reagent grade, 97%, powder

Sigma-Aldrich

无水碳酸钠, ACS reagent (primary standard), anhydrous, 99.95-100.05% dry basis

Sigma-Aldrich

氢氧化钠, pellets, semiconductor grade, 99.99% trace metals basis

Sigma-Aldrich

硫酸铵, ReagentPlus^®, ≥99.0%

Sigma-Aldrich

氢氧化钠, puriss. p.a., ACS reagent, reag. Ph. Eur., K ≤0.02%, ≥98%, pellets

SAFC

氯化钠溶液, 5 M

Sigma-Aldrich

无水碳酸钠, ReagentPlus^®, ≥99.5%