跳转至内容
Merck
  • Enantioselective high performance liquid chromatography and supercritical fluid chromatography separation of spirocyclic terpenoid flavor compounds.

Enantioselective high performance liquid chromatography and supercritical fluid chromatography separation of spirocyclic terpenoid flavor compounds.

Journal of chromatography. A (2014-07-22)
Mathias Schaffrath, Verena Weidmann, Wolfgang Maison
摘要

Chiral spirocyclic terpenoids are abundant natural flavors with significant impact particularly on the food industry. Chromatographic methods for analytical and preparative separation of these compounds are therefore of high interest to natural product chemists in academia and industry. Gas chromatography on chiral stationary phases is currently the standard method for the separation of volatile terpenoids, limiting the scale to analytical quantities. We report herein high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) protocols for the chiral separation of several racemic spirocyclic terpenoids such as the important flavors theaspirane and vitispirane. A screening of mobile phases and 16 commercially available chiral stationary phases (CSPs) largely based on polysaccharides led to identification of protocols for the separation of all terpenoids tested. SFC methods were found to be particularly useful for the separation of these spirocyclic flavors due to the volatility and low polarity of the compounds. The reported chiral HPLC and SFC protocols are scalable alternatives to gas chromatographic separations of volatile terpenoid flavors.

材料
货号
品牌
产品描述

Sigma-Aldrich
三氟乙酸, ReagentPlus®, 99%
Sigma-Aldrich
三氟乙酸, suitable for HPLC, ≥99.0%
Sigma-Aldrich
庚烷, suitable for HPLC, ≥99%
Sigma-Aldrich
庚烷, ReagentPlus®, 99%
Sigma-Aldrich
三氟乙酸, puriss. p.a., suitable for HPLC, ≥99.0% (GC)
Sigma-Aldrich
庚烷, HPLC Plus, for HPLC, GC, and residue analysis, 99%
Sigma-Aldrich
庚烷, puriss. p.a., reag. Ph. Eur., ≥99% n-heptane basis (GC)
Sigma-Aldrich
三氟乙酸, ≥99%, for protein sequencing
Sigma-Aldrich
庚烷, anhydrous, 99%
Sigma-Aldrich
庚烷, suitable for HPLC, ≥96%
Supelco
三氟乙酸, analytical standard
Supelco
庚烷, analytical standard
Supelco
庚烷, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
三氟乙酸, ReagentPlus®, 99%
Sigma-Aldrich
庚烷, biotech. grade, ≥99%