Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces.

Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces.

Current opinion in biotechnology (2013-04-02)

Leqian Liu, Heidi Redden, Hal S Alper

摘要

Microbial systems provide an attractive, renewable route to produce desired organic molecules such as fuels and chemicals. While attention within the field of metabolic engineering has mostly focused on Escherichia coli, yeast is a potent host and growing host for industrial products and has many outstanding, biotechnologically desirable native traits. Thus, there has been a recent shift in focus toward yeast as production hosts to replace E. coli. As such, products have diversified in yeast beyond simply ethanol. Additionally, nonconventional yeasts have been considered to move beyond Saccharomyces cerevisiae. This review highlights recent advances in metabolic engineering of yeasts for producing value-added chemical compounds including alcohols, sugar derivatives, organic acids, fats, terpenes, aromatics, and polyketides. Furthermore, we will also discuss the future direction of metabolic engineering of yeasts.

材料

货号

品牌

产品描述

E7023

Sigma-Aldrich

纯乙醇, 200 proof, for molecular biology

459844

Sigma-Aldrich

纯乙醇, 200 proof, ACS reagent, ≥99.5%

459828

Sigma-Aldrich

纯乙醇, 200 proof, HPLC/spectrophotometric grade

493546

Sigma-Aldrich

纯乙醇, 200 proof, meets USP testing specifications

E7148

Sigma-Aldrich

纯乙醇, 190 proof, for molecular biology

02870

Sigma-Aldrich

酒精, ACS reagent, prima fine spirit, without additive, F15 o¹

270741

Sigma-Aldrich

酒精试剂, suitable for HPLC

02856

Sigma-Aldrich

酒精, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA¹, ≥99.8% (based on denaturant-free substance)

362808

Sigma-Aldrich

酒精试剂, reagent grade

493511

Sigma-Aldrich

纯乙醇, 190 proof, ACS spectrophotometric grade, 95.0%

02857

Sigma-Aldrich

酒精, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL¹, ~96% (based on denaturant-free substance)

E-029

Supelco

乙醇-50标准液 CRM, 50 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

459836

Sigma-Aldrich

纯乙醇, 200 proof, anhydrous, ≥99.5%

48075

Supelco

Ethanol 溶液, certified reference material, 2000 μg/mL in methanol

32221

Sigma-Aldrich

酒精, puriss. p.a., absolute, ≥99.8% (GC)

E-030

Supelco

乙醇-80 标准液 CRM, 80 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-040

Supelco

乙醇-10标准液 CRM, 10 mg/dL in H₂O, pack of 10 × 1.2 mL ampules, certified reference material, Cerilliant^®

E-053

Supelco

乙醇-500标准液 CRM, 500 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-031

Supelco

乙醇-100（10支/盒)标准液 CRM, 100 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-033

Supelco

乙醇-300标准液 CRM, 300 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

02882

Sigma-Aldrich

酒精, purum, absolute ethanol, denaturated with 2% 2-butanone, A15 MEK¹, ≥99.8% (based on denaturant-free substance)

E2385

Supelco

10% (v/v) 乙醇标准品, 10 % (v/v) in H₂O, analytical standard

51976

Sigma-Aldrich

酒精, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

02890

Sigma-Aldrich

酒精, purum, fine spirit, denaturated with 2% 2-butanone, F25 MEK¹, ~96% (based on denaturant-free substance)

E-041

Supelco

乙醇-150, 150 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

1012772

USP

无水酒精, United States Pharmacopeia (USP) Reference Standard

E-032

Supelco

乙醇-200 (10支/盒)标准液 CRM, 200 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-056

Supelco

乙醇-20（10支/盒)标准液 CRM, 20 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-034

Supelco

乙醇校准套装, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-035

Supelco

Ethanol 溶液, 25 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®