- Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly.
Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly.
Bioelectrochemistry (Amsterdam, Netherlands) (2005-04-19)
M-L Calvo-Muñoz, A Dupont-Filliard, M Billon, S Guillerez, G Bidan, C Marquette, L Blum
PMID15833714
摘要
The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/pyrrole monomer ratio of 1/20,000.
材料