- The optimisation of a novel iodide microassay and its application in an immunoassay for human antibody levels in serum.
The optimisation of a novel iodide microassay and its application in an immunoassay for human antibody levels in serum.
Journal of immunological methods (1993-08-09)
R O'Kennedy, P Keating
PMID8354891
摘要
Radioactive iodine has been used as an antibody label in many immunoassays. The feasibility of using non-radioactive iodine as a label was investigated. A microassay for iodide based on the Sandell-Kolthoff reaction was optimised. It was used to detect iodine-labelled antibody and successfully applied to the detection of human IgG in serum samples. The performance of this assay system compared with enzyme-linked immunosorbent assay using HRP was evaluated.