跳转至内容
Merck
  • Anxiogenic effects of CGRP within the BNST may be mediated by CRF acting at BNST CRFR1 receptors.

Anxiogenic effects of CGRP within the BNST may be mediated by CRF acting at BNST CRFR1 receptors.

Behavioural brain research (2013-02-05)
K S Sink, A Chung, K J Ressler, M Davis, D L Walker
摘要

Calcitonin gene-related peptide (CGRP) acting within the bed nucleus of the stria terminalis (BNST) increases anxiety as well as neural activation in anxiety-related structures, and mediates behavioral stress responses. Similar effects have been described following intra-ventricular as well as intra-BNST infusions of the stress-responsive neuropeptide, corticotropin releasing factor (CRF). Interestingly, CGRP-positive terminals within the lateral division of the BNST form perisomatic baskets around neurons that express CRF, suggesting that BNST CGRP could exert its anxiogenic effects by increasing release of CRF from these neurons. With this in mind, the present set of experiments was designed to examine the role of CRFR1 signaling in the anxiogenic effects of CGRP within the BNST and to determine whether CRF from BNST neurons contributes to these effects. Consistent with previous studies, we found that 400 ng CGRP infused bilaterally into the BNST increased the acoustic startle response and induced anxiety-like behavior in the elevated plus maze compared to vehicle. Both of these effects were attenuated by 10mg/kg PO of the CRFR1 antagonist, GSK876008. GSK876008 alone did not affect startle. An intra-BNST infusion of the CRFR1 antagonist CP376395 (2 μg) also blocked increases in acoustic startle induced by intra-BNST infusion of CGRP, as did virally-mediated siRNA knockdown of CRF expression locally within the BNST. Together, these results suggest that the anxiogenic effects of intra-BNST CGRP may be mediated by CRF from BNST neurons acting at local CRFR1 receptors.

材料
货号
品牌
产品描述

Sigma-Aldrich
甲基纤维素, viscosity: 4,000 cP
Sigma-Aldrich
甲基纤维素, viscosity: 15 cP, BioReagent, suitable for cell culture
Sigma-Aldrich
甲基纤维素, viscosity: 1,500 cP
Sigma-Aldrich
甲基纤维素, viscosity: 15 cP
Sigma-Aldrich
甲基纤维素, viscosity: 25 cP
Sigma-Aldrich
甲基纤维素, 27.5-31.5% (Methoxyl content), viscosity: 400 cP
Sigma-Aldrich
甲基纤维素, meets USP testing specifications, 26.0-33.0% (methoxyl group, on Dry Basis), viscosity: 400 cP
Sigma-Aldrich
甲基纤维素, 26.0-33.0% (Methoxy group (dry basis)), meets USP testing specifications, viscosity: 1,500 cP
Sigma-Aldrich
Methocel® A15C, medium viscosity, Methoxyl content 27.5-31.5 %
Sigma-Aldrich
Methocel® A4M, viscosity 3000-5500 mPa.s, 2 % in H2O(20 °C)
Sigma-Aldrich
Methocel® A15 LV, 27.5-31.5% methoxyl basis
Sigma-Aldrich
甲基纤维素, tested according to Ph. Eur.