- A general method for detecting protease activity via gelation and its application to artificial clotting.
A general method for detecting protease activity via gelation and its application to artificial clotting.
Chemical communications (Cambridge, England) (2012-04-28)
Steven C Bremmer, Jing Chen, Anne J McNeil, Matthew B Soellner
PMID22538348
摘要
A modular system for detecting protease activity via enzyme-triggered gel formation is described. Protease-specific recognition sequences are utilized to achieve enzyme specificity. Artificial blood clotting is demonstrated by activating endogenous thrombin to trigger gelation in fibrinogen-deficient blood plasma.