- Construction of a homologous selectable marker gene for Lentinula edodes transformation.
Construction of a homologous selectable marker gene for Lentinula edodes transformation.
Bioscience, biotechnology, and biochemistry (2003-10-02)
Toshikazu Irie, Toshitsugu Sato, Kumiko Saito, Yoichi Honda, Takashi Watanabe, Masaaki Kuwahara, Hitoshi Enei
PMID14519992
摘要
We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.
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