Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP).

Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP).

Chemical communications (Cambridge, England) (2012-02-07)

Musleh M Muthana, Jingyao Qu, Yanhong Li, Lei Zhang, Hai Yu, Li Ding, Hamed Malekan, Xi Chen

摘要

A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further chemical diversification of a UDP-mannose derivative resulted in the formation of UDP-N-acetylmannosamine.

材料

货号

品牌

产品描述

I1643

Sigma-Aldrich

焦磷酸酶，无机来源于面包酵母（酿酒酵母）, powder, ≥500 units/mg protein (E1%/280)

I5907

Sigma-Aldrich

焦磷酸酶，无机来源于大肠杆菌, recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein

U8501

Sigma-Aldrich

尿苷-5′-二磷酸葡萄糖焦磷酸化酶来源于面包酵母, Type X, lyophilized powder, ≥40 units/mg protein

G6048

Sigma-Aldrich

半乳糖激酶人, recombinant, expressed in E. coli