Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines.

Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines.

Stem cell research (2021-02-22)

C H Cole Sims, Matias I Autio, Adriana Buskin, Christine Cheung, Rakesh Heer, Roger S Y Foo, Xin Wang

摘要

Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.