跳转至内容
Merck
  • Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division.

Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division.

Science advances (2021-06-13)
Xiang Zhao, Jason Q Garcia, Kai Tong, Xingye Chen, Bin Yang, Qi Li, Zhipeng Dai, Xiaoyu Shi, Ian B Seiple, Bo Huang, Su Guo
摘要

In the developing embryos, the cortical polarity regulator Par-3 is critical for establishing Notch signaling asymmetry between daughter cells during asymmetric cell division (ACD). How cortically localized Par-3 establishes asymmetric Notch activity in the nucleus is not understood. Here, using in vivo time-lapse imaging of mitotic radial glia progenitors in the developing zebrafish forebrain, we uncover that during horizontal ACD along the anteroposterior embryonic axis, endosomes containing the Notch ligand DeltaD (Dld) move toward the cleavage plane and preferentially segregate into the posterior (subsequently basal) Notchhi daughter. This asymmetric segregation requires the activity of Par-3 and dynein motor complex. Using label retention expansion microscopy, we further detect Par-3 in the cytosol colocalizing the dynein light intermediate chain 1 (Dlic1) onto Dld endosomes. Par-3, Dlic1, and Dld are associated in protein complexes in vivo. Our data reveal an unanticipated mechanism by which cytoplasmic Par-3 directly polarizes Notch signaling components during ACD.

材料
货号
品牌
产品描述

Sigma-Aldrich
抗-豚鼠 IgG(全分子)-过氧化物酶 兔抗, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
酚红 溶液, 0.5%, liquid, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
抗-小鼠-IgG - Atto 647N 山羊抗, contains 50% glycerol as stabilizer