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  • 3D Printed Stem-Cell Derived Neural Progenitors Generate Spinal Cord Scaffolds.

3D Printed Stem-Cell Derived Neural Progenitors Generate Spinal Cord Scaffolds.

Advanced functional materials (2018-09-26)
Daeha Joung, Vincent Truong, Colin C Neitzke, Shuang-Zhuang Guo, Patrick J Walsh, Joseph R Monat, Fanben Meng, Sung Hyun Park, James R Dutton, Ann M Parr, Michael C McAlpine
摘要

A bioengineered spinal cord is fabricated via extrusion-based multi-material 3D bioprinting, in which clusters of induced pluripotent stem cell (iPSC)-derived spinal neuronal progenitor cells (sNPCs) and oligodendrocyte progenitor cells (OPCs) are placed in precise positions within 3D printed biocompatible scaffolds during assembly. The location of a cluster of cells, of a single type or multiple types, is controlled using a point-dispensing printing method with a 200 μm center-to-center spacing within 150 μm wide channels. The bioprinted sNPCs differentiate and extend axons throughout microscale scaffold channels, and the activity of these neuronal networks is confirmed by physiological spontaneous calcium flux studies. Successful bioprinting of OPCs in combination with sNPCs demonstrates a multicellular neural tissue engineering approach, where the ability to direct the patterning and combination of transplanted neuronal and glial cells can be beneficial in rebuilding functional axonal connections across areas of central nervous system (CNS) tissue damage. This platform can be used to prepare novel biomimetic, hydrogel-based scaffolds modeling complex CNS tissue architecture in vitro and harnessed to develop new clinical approaches to treat neurological diseases, including spinal cord injury.

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Sigma-Aldrich
海藻酸 钠盐 来源于褐藻, low viscosity
Sigma-Aldrich
海藻酸 钠盐 来源于褐藻, Medium viscosity