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Merck
  • Cerium elicitor-induced phosphatidic acid triggers apoptotic signaling development in Taxus cuspidata cell suspension cultures.

Cerium elicitor-induced phosphatidic acid triggers apoptotic signaling development in Taxus cuspidata cell suspension cultures.

Chemistry and physics of lipids (2009-05-12)
Song Yang, Shu-Huan Lu, Ying-Jin Yuan
摘要

Degradation of membrane phospholipids is associated with apoptotic responses, but the signaling development of this degradation is not well understood. Cerium (Ce(4+)), an important rare earth element, induces cellular apoptosis and taxol biosynthesis in Taxus cuspidata suspension cultures. Here, using mass spectrometry and biochemical technique, we demonstrated that the phospholipase D (PLD) was rapidly activated by Ce(4+) and hydrolyzed structural phospholipids to generate lipid signal molecule, phosphatidic acid (PA). 1-Butanol, an antagonist of PLD-dependent PA production, blocked the biphasic burst of superoxide anions (O2(*-)) and thus mitigated cellular apoptosis. The time-course analysis of PA accumulation and ERK-like mitogen-activated protein kinase (MAPK) regulation indicated PA generation preceded MAPK activation, suggesting that the rapid accumulation of PA might be required for the initial MAPK activity. After 2h of Ce(4+) elicitation, however, PA-induced O2(*-) burst, forming a negative regulation to MAPK activity, which in turn led to apoptotic signaling development.

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Avanti
16:0 Phosphatidylbutanol, 1,2-dipalmitoyl-sn-glycero-3-phosphobutanol (sodium salt), chloroform