An improved rapid method for the preparation of D-rhamnose.

An improved rapid method for the preparation of D-rhamnose.

Carbohydrate research (2011-11-22)

Olga N Smol'kina, Natalya S Shishonkova, Yulia P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Vladimir V Ignatov

PMID22099380

摘要

A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was crystallized. The sugar activity was demonstrated immunochemically. The advantages of the method are that it expedites and simplifies the extraction of D-rhamnose and increases its yield.