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  • Beclin1 restricts RNA virus infection in plants through suppression and degradation of the viral polymerase.

Beclin1 restricts RNA virus infection in plants through suppression and degradation of the viral polymerase.

Nature communications (2018-03-30)
Fangfang Li, Changwei Zhang, Yinzi Li, Guanwei Wu, Xilin Hou, Xueping Zhou, Aiming Wang
摘要

Autophagy emerges as an essential immunity defense against intracellular pathogens. Here we report that turnip mosaic virus (TuMV) infection activates autophagy in plants and that Beclin1 (ATG6), a core component of autophagy, inhibits virus replication. Beclin1 interacts with NIb, the RNA-dependent RNA polymerase (RdRp) of TuMV, via the highly conserved GDD motif and the interaction complex is targeted for autophagic degradation likely through the adaptor protein ATG8a. Beclin1-mediated NIb degradation is inhibited by autophagy inhibitors. Deficiency of Beclin1 or ATG8a enhances NIb accumulation and promotes viral infection and vice versa. These data suggest that Beclin1 may be a selective autophagy receptor. Overexpression of a Beclin1 truncation mutant that binds to NIb but lacks the ability to mediate NIb degradation also inhibits virus replication. The Beclin1-RdRp interaction further extends to several RNA viruses. Thus Beclin1 restricts viral infection through suppression and also likely autophagic degradation of the viral RdRp.

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Sigma-Aldrich
抗-HA 兔抗, affinity isolated antibody, buffered aqueous solution