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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3B13, recombinant monoclonal
描述
3B13 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 21.3 kDa
observed mol wt ~20 kDa
純化經由
using Protein A
物種活性
mouse, rat, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
ELISA: suitable
affinity binding assay: suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... HRAS(3265)
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 3B12 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects H-Ras. It targets an epitope within 18 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human H-Ras.
應用
Quality Control Testing
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected H-Ras in Human brain tissue lysate
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected H-Ras in KNRK and Mouse Brain lysates.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected full-length recombinant Human H-Ras.
Affinity Binding Assay:: A representative lot of this antibody bound H-Ras with a KD of 1.9 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected H-Ras in Human brain tissue lysate.
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected H-Ras in Human brain tissue lysate
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected H-Ras in KNRK and Mouse Brain lysates.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected full-length recombinant Human H-Ras.
Affinity Binding Assay:: A representative lot of this antibody bound H-Ras with a KD of 1.9 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected H-Ras in Human brain tissue lysate.
標靶描述
GTPase HRas (UniProt: P01112; also known as EC:3.6.5.2, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras) is encoded by the HRAS (also known as HRAS1) gene (Gene ID: 3265) in human. The RAS oncogene family consists of three members, HRAS, KRAS, and NRAS that encode membrane-associated proteins involved in mediation of signals arising from cell-membrane receptor tyrosine kinases and G-protein-coupled receptors along the MAPK, PI3K/AKT and other signaling pathways. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. H-Ras is widely expressed small G-protein that is synthesized with a propeptide (aa 187-189), which is subsequently cleaved off to produce the mature form that alternates between an inactive form bound to GDP and an active form bound to GTP. It is activated by a guanine nucleotide-exchange factor (GEF) and is inactivated by a GTPase-activating protein (GAP). The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form. Acetylation at lysine 104 is reported to prevent interaction with GEFs. H-Ras is known to undergo palmitoylation at cysteines 181 and 184, directly upstream of the CAAX motif, as the final step of its activation prior to membrane localization. H-Ras contains a highly conserved G domain (aa 1-165) and a hypervariable region (aa 166-185). The G domain contains the vital switch I and II domains within its effector lobe (aa 1-86), an area that is conserved across all Ras isoforms. This region is responsible for allowing Ras to interact with effector protein pathways, primarily Raf-MEK-ERK and PI3K/AKT, to mediate cell growth, survival, and proliferation. This domain also the most common sites (G12, G13, and Q61) of Ras oncogenic mutations. The three RAS genes account for the most frequently mutated oncogene family in human cancers. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shu, L., et al. (2020). Mol. Cancer Ther. 19(4); 999-1007).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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