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生物源
human
重組細胞
expressed in E. coli
化驗
≥98% (HPLC)
≥98% (SDS-PAGE)
形狀
lyophilized
效力
1.0-10.0 ng/mL
分子量
7.8 kDa
包裝
pkg of 20 μg
雜質
<0.1 EU/μg endotoxin, tested
顏色
white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... CCL5(6352)
一般說明
Regulated on activation, normal T cell expressed and secreted (RANTES) also known as C-C motif chemokine ligand 5 (CCL5) is encoded by the gene mapped to human chromosome 17. RANTES belongs to intercrine or chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections. Recombinant human RANTES is a 7.8kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
應用
RANTES human has been used in following studies:
- In transmigration experiments to determine whether LFA-1 cross-linking could affect peripheral blood T lymphocytes (PBTL) migration in response to the chemokine RANTES.
- Luminol-dependent chemiluminescence assay to determine the effect of RANTES on reactive oxygen species (ROS) production by eosinophils.
生化／生理作用
Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4+/CD45RO), basophils, and eosinophils. The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5.1 RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. The In1.1C variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV-1 infection.
外觀
Lyophilized with no additives.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
RANTES-activated human T lymphocytes. A role for phosphoinositide 3-kinase.
Turner L
Journal of Immunology, 155(5), 2437-2444 (1995)
Role of chemokine and cytokine polymorphisms in the progression of HIV-1 disease.
Mahajan SD
Biochemical and Biophysical Research Communications, 396(2), 348-352 (2010)
J J Oppenheim et al.
Annual review of immunology, 9, 617-648 (1991-01-01)
A family consisting of at least ten distinct novel 8-10 kd cytokines has been identified over the past 12 years. These cytokines exhibit from 20 to 45% homology in amino acid sequence, are probably all basic heparin-binding polypeptides, and have
Inside the crawling T cell: leukocyte function-associated antigen-1 cross-linking is associated with microtubule-directed translocation of protein kinase C isoenzymes beta(I) and delta.
Volkov Y
Journal of Immunology, 161(12), 6487-6495 (1998)
A role for the chemokine RANTES in regulating CD8 T cell responses during chronic viral infection.
Crawford A
PLoS Pathogens, 7(7), e1002098-e1002098 (2011)
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