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Merck

SEP080

Millipore

Seppro® Rubisco

LC2

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

色谱柱直径 × 长度

6.4 mm × 63 mm

容量

1 mg total protein loading(D-Ribulose 1,5-Diphosphate Carboxylase (Rubisco))

储存温度

2-8°C

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一般描述

RuBisCO(核酮糖-1,5-二磷酸羧化酶/加氧酶)是植物中最丰富的蛋白质,而且可能也是地球上最丰富的蛋白质。RuBisCO是一种酶,在Calvin循环中催化碳固定反应的第一个关键步骤,通过碳固定反应可将大气中游离的二氧化碳转化为生物体内储能分子,如蔗糖分子。RuBisCO是绿叶进行光合碳同化的关键酶,同时也是植物蛋白组学研究的主要障碍。RuBisCO约占绿叶中蛋白总量的40%,可干扰蛋白质组学研究,如LC-MS/MS和二维凝胶电泳。为了从其他植物蛋白中特异和有效分离RuBisCO,Sigma开发了基于IgY抗体与微球交联的新型免疫亲和离心柱,可特异性去除植物提取物中的RuBisCO蛋白。

应用

Seppro® Rubisco,LC2(2ml 柱)可用于植物蛋白质组学研究,采用IgY介导的免疫亲和性分离,通过液相色谱法实现从植物组织提取物中去除RuBisCO(核酮糖-1,5-二磷酸羧化酶/加氧酶)。

法律信息

Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

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产品编号
说明
化学品安全说明书

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  • S4324Seppro® Stripping Buffer化学品安全说明书

  • S4449Seppro® Neutralization Buffer化学品安全说明书

  • CLS8163Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterile化学品安全说明书

储存分类代码

10 - Combustible liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Renato Millioni et al.
PloS one, 6(5), e19603-e19603 (2011-05-17)
To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number

Questions

1–5 of 5 Questions  
  1. How can I extract my plant proteins for use with Seppro® Rubisco kit, Product SEP080?

    1 answer
    1. One suggestion is to use PBS with 0.05% Tween 20. Extraction with urea/SDS is not suggested because this is incompatible with the antibodies in the Seppro products.

      Helpful?

  2. I originally purchased my Seppro RuBisCO LC2 column from Genway. Can I still contact Genway for technical support?

    1 answer
    1. All inquiries on the Seppro technology are now to be referred to Sigma-Aldrich. Customers should not contact Genway for technical assistance.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Can I do TCA/acetone precipitation for isolating plant proteins to use with the Seppro® Rubisco kit, Product SEP080?

    1 answer
    1. TCA/acetone precipitation is problematic because it may be difficult to redissolve the protein pellet afterwards. The following paper provides some tips for extraction: Cellar, N.A., et al., J. Chromatography B, 861, 29-39 (2008).

      Helpful?

  5. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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