生物源
rabbit
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
human, mouse, rat
技術
microarray: suitable
western blot: 1:2,000 using a cytosolic fraction of rat brain.
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... LIMK1(3984)
mouse ... Limk1(16885)
rat ... Limk1(65172)
一般說明
LIM Domain Kinase 1 (LIMK1) belongs to the LIMK family of serine/threonine protein kinases. LIMK1 shares about 50% sequence identity with LIMK2. LIMK1 contains two N-terminal LIM domains, an internal PDZ (post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), and zonula occludens-1 protein (zo-1)) domain and a C-terminal kinase domain.
免疫原
Synthetic peptide corresponding to the C-terminus of mouse LIMK1 (amino acids 627-647 or 613-633 of the alternatively spliced forms of LIMK1), conjugated to KLH
This sequence is identical in rat LIMK1 and highly conserved (single amino acid substitution) in human LIMK1. It is not found in the LIMK2 isoform.
This sequence is identical in rat LIMK1 and highly conserved (single amino acid substitution) in human LIMK1. It is not found in the LIMK2 isoform.
應用
Anti-LIM Kinase 1 antibody is suitable for use in microarrays, protein densitometric assays (1:1000 using BPAE cell extracts ) and western blot (1:100, 000 using MDA-MB-231 cell lysates ).
生化／生理作用
LIMK1 phosphorylates cofilin specifically at Serine 3. This inactivates its F-actin depolymerizing activity and leads to actin reorganization and accumulation of actin filaments. LIMK1 mRNA is highly expressed in the developing nervous system, heart and gut, and in adult brain and spinal cord, suggesting a role for LIMK-1 during neuronal cell differentiation. LIMK1 is localized in the presynaptic nerve ending during the synapse maturation event. LIMK1 also exhibits mitosis-specific activation.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Tissue specific characterisation of Lim-kinase 1 expression during mouse embryogenesis
Lindstrom NO, et al.
Gene Expression Patterns, 11(3-4), 221-232 (2011)
Mitosis-specific activation of LIM motif-containing protein kinase and roles of cofilin phosphorylation and dephosphorylation in mitosis
Amano T, et al.
The Journal of biological chemistry, 277(24), 22093-22102 (2002)
Patricia Castañeda et al.
Journal of neuroscience research, 93(10), 1476-1491 (2015-05-27)
Chronic stress promotes cognitive impairment and dendritic spine loss in hippocampal neurons. In this animal model of depression, spine loss probably involves a weakening of the interaction between pre- and postsynaptic cell adhesion molecules, such as N-cadherin, followed by disruption
Zoe M Goeckeler et al.
The Journal of biological chemistry, 280(38), 33083-33095 (2005-08-02)
This study determined the effects of increased intracellular cAMP and cAMP-dependent protein kinase activation on endothelial cell basal and thrombin-induced isometric tension development. Elevation of cAMP and maximal cAMP-dependent protein kinase activation induced by 10 microm forskolin, 40 microm 3-isobutyl-1-methylxanthine
K Ohashi et al.
The Journal of biological chemistry, 275(5), 3577-3582 (2000-02-01)
LIM-kinase 1 (LIMK1) phosphorylates cofilin, an actin-depolymerizing factor, and regulates actin cytoskeletal reorganization. LIMK1 is activated by the small GTPase Rho and its downstream protein kinase ROCK. We now report the site of phosphorylation of LIMK1 by ROCK. In vitro
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