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種類
Type I
形狀
ammonium sulfate suspension
比活性
200-500 units/mg protein
異物活動
glutamic-pyruvic transaminase ≤0.03%
lactic dehydrogenase ≤0.01%
malic dehydrogenase ≤0.01%
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
谷草转氨酶见于所有组织，主要见于肝脏和骨骼肌。它是折叠型I型吡哆醛5′-磷酸(PLP)酶的原型。谷草转氨酶是一种同型二聚体，在每个亚基中含有大小结构域。
應用
来自猪心脏的谷草转氨酶已用于：
- 在酶偶联试验中评价L-天冬酰胺酶活性
- 在微量滴定板格式酶基础检测中估算浆果样品中苹果酸的含量
- 作为均质缓冲液的补充，用于分离草酰乙酸(OAA)消耗系统环境下的心脏线粒体膜
Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.
生化／生理作用
谷草转氨酶或天冬氨酸转氨酶(AAT)催化L-天冬氨酸和α-酮戊二酸与草乙酸和L-谷氨酸的相互转化。这种可逆的转氨酶反应依赖于5′-磷酸吡哆醛(PLP)。谷草转氨酶通过产生L-谷氨酸维持代谢的氮。血清谷草转氨酶水平升高在心肌梗死、肝脏疾病和某些肾脏疾病中都有指示意义。
單位定義
一个酶活性单位是指在pH 7.5、37 °C下，在 L-天冬氨酸存在时，每分钟将1.0 μmole的α-酮戊二酸转化为L-谷氨酸所需的酶量。在25 °C下，一个单位相当于约2,000 O.D.（Karmen）单位。
外觀
含 0.05M 马来酸盐和 2.5mM α-酮戊二酸盐的 3.0M (NH4)2SO4 中的悬浮液，pH 6.0
分析報告
由缩二脲确定的蛋白质。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Clinical biochemistry and hematology
The laboratory rabbit, guinea pig, hamster, and other rodents., 57-116 (2012)
Anna Stepanova et al.
Biochimica et biophysica acta, 1857(9), 1561-1568 (2016-06-12)
Mitochondrial Complex II is a key mitochondrial enzyme connecting the tricarboxylic acid (TCA) cycle and the electron transport chain. Studies of complex II are clinically important since new roles for this enzyme have recently emerged in cell signalling, cancer biology
The Study of Serum GOT (Glutamic Oxalacetic Transaminase) Activity and Some kinetic Parameters in Patient with Pulmonary Tuberculosis
Ismahil PA and Hassan LM
Biological Chemistry, 23, 159-169 (2017)
Pyridoxal 5-phosphate-dependent enzymes: catalysis, conformation, and genomics
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Archives of biochemistry and biophysics, 544, 119-127 (2013-10-15)
Aspartate aminotransferase (AAT) is a prototypical pyridoxal 5'-phosphate (PLP) dependent enzyme that catalyzes the reversible interconversion of l-aspartate and α-ketoglutarate with oxalacetate and l-glutamate via a ping-pong catalytic cycle in which the pyridoxamine 5'-phosphate enzyme form is an intermediate. There
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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