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Merck

F6636

SAFC

营养混合物 F-12 Ham

Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

别名:

Ham's F-12

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

营养混合物 F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture

形狀

powder

技術

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

成分

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

運輸包裝

ambient

儲存溫度

2-8°C

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應用

库恩对哈姆氏 F-12 的改造是为培养通过病毒融合产生的杂交细胞而开发的。修饰包括使氨基酸和丙酮酸加倍,并包括抗坏血酸。盐浓度也发生了改变。配方中含有 0.863 mg/L 硫酸锌,这可能使其不适合培养小鼠 L -细胞。

數量

按照每升培养基11.5克粉末配制。

重構

补充2.68 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Xiaojiao Xue et al.
Human molecular genetics, 26(16), 3116-3129 (2017-06-03)
In-frame premature termination codons (PTCs) account for ∼11% of all disease-associated mutations. PTC suppression therapy utilizes small molecules that suppress translation termination at a PTC to restore synthesis of a full-length protein. PTC suppression is mediated by the base pairing
Feng Liang et al.
SLAS technology, 22(3), 315-324 (2017-04-06)
Cystic fibrosis (CF) is a hereditary disease caused by mutations in the gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR). A large number of nearly 2000 reported mutations, including the premature termination codon (PTC) mutations, urgently require new
Kenneth A Giuliano et al.
SLAS discovery : advancing life sciences R & D, 23(2), 111-121 (2017-09-13)
Cystic fibrosis (CF) is a lethal genetic disorder caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Despite recent groundbreaking approval of genotype-specific small-molecule drugs, a significant portion of CF patients still lack effective therapeutic options that
Wren E Michaels et al.
Nucleic acids research, 48(13), 7454-7467 (2020-06-11)
Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, encoding an anion channel that conducts chloride and bicarbonate across epithelial membranes. Mutations that disrupt pre-mRNA splicing occur in >15% of
Nyla Naim et al.
Methods in molecular biology (Clifton, N.J.), 2173, 201-216 (2020-07-12)
cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target

实验方案

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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