RO82-W-1

92030502, human thyroid, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: RO82-W-1, 92030502
• 生物源: human thyroid
• 增長模式: Adherent
• 染色體組型: 2n = 60-82, 82 predominant
• 形態學: Epithelial
• 產品: Thyroglobulin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human follicular thryoid carcinoma

細胞系描述

Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.

應用

A valuable model for the study of human follicular carcinoma.

培養基

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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