Fc3Tg

90073002, feline embryonic tongue. Fibroblast-like morphology

• 分類程式碼代碼: 41106514

属性

• product name: Fc3Tg, 90073002
• 生物源: feline mouth
• 增長模式: Adherent
• 染色體組型: 2n = 38
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Cat tongue

細胞系描述

Established from normal embryonal tongue tissue of female cat (Felis catus). Cells support the replication of a wide range of feline viruses. Can undergo 50 population doublings.

應用

Virus replication studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂ 37°C. Maintain at pH 7.1-7.3.

其他說明

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