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product name
HL60 M4, 88120804
生物源
human blood
增長模式
Suspension
染色體組型
Modal no. 45
形態學
Not specified
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human Caucasian promyelocytic leukaemia
細胞系描述
HL60 induced to mature into neutrophils with 1.75% DMSO after treating with SV40. Fails to express SV40 specific T Ag suggesting that HL60 M4 represents expansion of sub-clones of HL60 with inherent restricted capacity for neutrophil differentiation.
應用
Differentiation studies
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml. Do not allow the culture medium to become acidic or the cells will differentiate; 5% CO2; 37°C.
其他說明
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