MM138 Cell Line human

10092321, human skin, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: MM138 Cell Line human, 10092321
• 生物源: human skin
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human melanoma; from metastatic site - lymph node

細胞系描述

Metastatic Melanoma (metastatic site: lymph node). Cell Bank Australia reference number: CBA1345.

培養基

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% fetal bovine serum (FBS).

例行更新培養

Optimal split ratio 1:4, MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard the doubling time is greatly increased. (Seeding density 0.8 x10⁴ cells/cm²). Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.