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Merck

T9285

Sigma-Aldrich

Tris-EDTA 缓冲液

100X concentrate for Northern and Southern blotting

别名:

Tris-EDTA洗涤液, TE 缓冲液 溶液

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About This Item

MDL號碼:
分類程式碼代碼:
41105319
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

品質等級

無菌

0.2 μm filtered

形狀

liquid

pH值

7.8-8.2 (1 × in water)

應用

sample preparation

異物活動

DNase, RNase, none detected

儲存溫度

room temp

SMILES 字串

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI 密鑰

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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一般說明

Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物(包括DNA和RNA)的重悬和保存。其中,Tris-HCl是常用缓冲液,EDTA是金属螯合剂。TE缓冲液可溶解核酸,同时保护其免受降解。可用于核酸分离,比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。

應用

TE缓冲液已用于:
  • 分离、重悬或保存DNA和RNA用于PCR或qPCR
  • 凝胶电泳,包括Northern和Southern免疫印迹分析
  • 细菌细胞,用于通过AmpC纸片试验检测细胞释放的β-内酰胺酶
  • 免疫组化的抗原修复

成分

TE缓冲液包含1M Tris-HCl(pH约8.0)和0.1M EDTA。

其他說明

如需了解生化试剂系列的更多信息,请填写此表

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
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AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)

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