MABE305

Anti-Dnmt3b Antibody, clone D3B2-2C1

clone D3B2-2C1, from rat

• 别名: DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: D3B2-2C1, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_001193984
• UniProt登錄號: Q9UBC3
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DNMT3B(1789)

说明

一般說明

DNA (cytosine-5)-methyltransferase 3B (Dnmt3B) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. The Dnmt3b enzyme is involved in de novo methylation and the establishment of genome-wide DNA methylation patterns during development. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.

免疫原

Linear peptide corresponding to human Dnmt3b.

應用

Anti-Dnmt3b Antibody, clone D3B2-2C1 is a Rat Monoclonal Antibody for detection of Dnmt3b also known as DNA (cytosine-5)-methyltransferase 3B & has been validated in WB & IP.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt3b in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

品質

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Dnmt3b in 10 µg of of HeLa nuclear extract.

標靶描述

~110 kDa observed. Multiple isoforms between ~80-96 kDa are known to exist. A truncated protein at ~40 kDa may be observed in some cell lysates (Ostler, K. R., et al. (2007). 26(38): 5553-5563.). Uncharacterized bands may be observed at ~20 kDa and ~34 kDa in some cell lysates.

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa nuclear extract

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable